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Gönül Schara

Contact Information

(209) 667-3583
gschara@csustan.edu

Naraghi Hall of Science, N354

College of Science

Journal Publications

  1. Gönül Vardar and Thomas K. Wood “Protein Engineering of Toluene-o-Xylene Monooxygenase from Pseudomonas stutzeri OX1 for Synthesizing 4-Methylresorcinol, Methylhydroquinone, and Pyrogallol” Applied and Environmental Microbiology 70: 3253-3262, 2004.
  2. Gönül Vardar, Kang Ryu, and Thomas K. Wood “Protein Engineering of Toluene-o-Xylene Monooxygenase from Pseudomonas stutzeri OX1 for Oxidizing Nitrobenzene to 3-Nitrocatechol, 4-Nitrocatechol, and Nitrohydroquinone” Journal of Biotechnology 115: 145-156, 2005.
  3. Gönül Vardar, Paola Barbieri, and Thomas K. Wood. “Chemotaxis of Pseudomonas stutzeri OX1 and Bulkholderia cepacia G4 toward chlorinated ethenes” Applied Microbiology and Biotechnology 66: 696-701, 2005.
  4. Gönül Vardar and Thomas K. Wood “Protein Engineering of Toluene-o-Xylene Monooxygenase from Pseudomonas stutzeri OX1 for Enhanced Chlorinated Ethene Degradation and o-Xylene Oxidation” Applied Microbiology and Biotechnology 68: 510-517, 2005.
  5. Gönül Vardar and Thomas K. Wood “Alpha-Subunit Positions Methionine 180 and Glutamate 214 of Pseudomonas stutzeri OX1 Toluene-o-Xylene Monooxygenase Influence Catalysis” Journal of Bacteriology 187: 1511-1514, 2005.
  6. Gönül Vardar, Jintae Lee, Ying Tao, and Thomas K. Wood “Alpha Subunit Positions Alanine 101 and Alanine 110 of Pseudomonas stutzeriOX1 Toluene-o-Xylene Monooxygenase Influence the Regiospecific Oxidation of Aromatics” Biotechnology and Bioengineering 92: 652-658, 2005.
  7. Toshinari Maeda, Gönül Vardar, William T. Self, and Thomas K. Wood “Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803” BMC Biotechnology 7: 25, 2007.
  8. Gönül Vardar-Schara, Ivo M. Krab, Guohua Yi, Wei Wen Su “A Homogeneous Fluorometric Assay Platform Based on Novel Synthetic Proteins” Biochemical and Biophysical Research Communications 361: 103-108, 2007.
  9. Gönül Vardar-Schara, Toshinari Maeda, and Thomas K. Wood “Metabolically engineered bacteria for producing hydrogen via fermentation” Microbial Biotechnology 1:107-125, 2008.
  10. Nurcan Vardar and Gönül Vardar-Schara. “Fosmid Cloning, Nucleotide Sequence, and Characterization of a Beta-Lactamase Gene from Subsurface Isolates” Journal of Microbiology 50: 680-683, 2012.
  11. Burcu Sonmez, K. Cansu Yanık, Thomas K. Wood, and Gönül Vardar-Schara “The Role of Substrate Binding Pocket Residues Phenylalanine 176 and Phenylalanine 196 on Pseudomonas sp. OX1 Toluene o-Xylene Monooxygenase Activity and Regiospecificity” Biotechnology and Bioengineering 111: 1506-1512, 2014.
  12. K. Cansu Yanık and Gönül Vardar-Schara “Saturation mutagenesis of Bradyrhizobium sp. BTAi1 toluene 4-monooxygenase at alpha-subunit residues proline 101, proline 103, and histidine 214 for regiospecific oxidation of aromatics” Applied Microbiology and Biotechnology 98: 8975-8986, 2014.
  13. Cansu Kurt, Burcu Sonmez, Nurcan Vardar, K. Cansu Yanık-Yıldırım, Gönül Vardar-Schara “Cavity Residue Leucine 95 and Channel Residues Glutamine 204, Aspartic Acid 211, and Phenylalanine 269 of Toluene o-Xylene Monooxygenase Influence Catalysis” Applied Microbiology and Biotechnology 100: 7599-7609, 2016.
  14. Fatma Feyza Özgen, Nurcan Vardar-Yel, Owen S Roth, Lersa S Shahbaz, Gönül Vardar-Schara “Surface Residues Serine 69 and Arginine 194 of Metagenome-Derived Lipase Influence Catalytic Activity” Biochemical Engineering Journal, 154: 107442, 2020.
  15. K Cansu Yanık-Yıldırım, Onkar K Phul, Owen S Roth, Areli Tlatelpa, Gerardo Soria-P, Nurcan Vardar-Yel, Gönül Vardar-Schara “Regiospecific Oxidation of Chlorobenzene to 4-Chlororesorcinol, Chlorohydroquinone, 3-Chlorocatechol and 4-Chlorocatechol by Engineered Toluene o-Xylene Monooxygenases” Applied and Environmental Microbiology 2022 Jul 12; 88(13): e0035822. doi: 10.1128/aem.00358-22.

Book Chapter

  1. Ayelet Fishman, Ying Tao, Gönül Vardar, Lingyun Rui, and Thomas K. Wood “Controlling Regiospecific Oxidation of Aromatics and the Degradation of Chlorinated Aliphatics via Active Site Engineering of Toluene Monooxygenases,” Pseudomonas 4: Molecular Biology of Emerging Issues. p 237-286, 2006.

Abstracts and Proceedings

  1. Gönül Vardar and Thomas K. Wood. Directed Evolution of Toluene-o-Xylene Monooxygenase from Pseudomonas stutzeri OX1 for the degradation of chlorinated aliphatics. ACS Annual Meeting, Boston, MA, USA, 2002.
  2. Gönül Vardar and Thomas K. Wood. Directed Evolution of Toluene-o-Xylene Monooxygenase from Pseudomonas stutzeri OX1 for the Production of 4-Methylresorcinol, Methylhydroquinone, and Pyrogallol. 227th ACS National Meeting, Anaheim, CA, USA, 2004.
  3. Gönül Vardar, Paola Barbieri, and Thomas K. Wood. Chemotaxis of Pseudomonas stutzeri OX1 and Bulkholderia cepacia G4 toward chlorinated ethenes. ASM 104th General Meeting, New Orleans, Louisiana, 2004.
  4. Gönül Vardar and Thomas K. Wood. Protein Engineering of Toluene-o-Xylene Monooxygenase from Pseudomonas stutzeri OX1 for the Production of 4-Methylresorcinol, Methylhydroquinone, Pyrogallol, 1,2,4-Trihydroxybenzene, Nitrohydroquinone, 3-, and 4-Nitrocatechol. ASM 104th General Meeting, New Orleans, Louisiana, 2004.
  5. Gönül Vardar-Schara. A Self-reporting Biosensing Platform based on Novel Synthetic Proteins. 6th Nanoscience and Nanotechnology Conference (NanoTR-VI), Izmir, Turkey, 2010. 
  6. Gönül Vardar-Schara. Protein Engineering of Toluene-o-Xylene Monooxygenase for Green Chemistry. Vth International Bioengineering Congress, Izmir, Turkey, 2010.
  7. Nurcan Vardar and Gönül Vardar-Schara. A Novel Beta-lactamase from Subsurface Sediments. Current Opinion in Biotechnology 22: S123-S124, 2011, European Biotechnology Congress 2011.
  8. K. Cansu Yanik and Gönül Vardar-Schara. Cloning and characterization of a gene cluster encoding toluene monoxygenase from Bradyrhizobium sp. BTAi1 for green chemistry and bioremediation. 6th International Congress on Biocatalysis, P174, Hamburg, Germany, 2012.
  9. Burcu Sonmez, K. Cansu Yanik, Thomas K. Wood, and Gönül Vardar-Schara. The Role of Substrate Binding Pocket Residues Phenylalanine 176 and Phenylalanine 196 on Pseudomonas sp. OX1 Toluene o-Xylene Monooxygenase Activity and Regiospecificity. 7th International Congress on Biocatalysis, Hamburg, Germany, 2014.
  10. Cansu Kurt, Burcu Sönmez, Nurcan Vardar-Yel, K. Cansu Yanık-Yıldırım, and Gönül Vardar-Schara. The Role of Cavity Residue Leucine 95 and Channel Residues Glutamine 204, Aspartic Acid 211, and Phenylalanine 269 on Toluene-o-xylene Monooxygenase Activity and Regiospecificity. Enzyme Engineering XXV Conference, Whistler, Canada, 2019.
  11. Onkar K. Phul, Owen S. Roth, Gerardo Soria-P, and Gönül Vardar-Schara. Oxidation of chlorobenzene to 4-chlororesorcinol, chlorohydroquinone, 3-chlorocatechol and 4-chlorocatechol by toluene o-xylene monooxygenase. 32nd Annual CSU Biotechnology Symposium, Santa Clara, CA, 2020.
  12. Owen S. Roth, Onkar K. Phul, Areli Tlatelpa, and Gönül Vardar-Schara. Regiospecific oxidation of chlorobenzene by toluene monooxygenases and engineered toluene o-xylene monooxygenases of Pseudomonas sp. OX1. 32nd Annual CSU Biotechnology Symposium, Santa Clara, CA, 2020.
  13. John Molthen and Gönül Vardar-Schara. Designing an oxygenase enzyme: from benchtop to laptop. CSU CURES Network Fall 2020 Mini-Symposium on "Virtual Cures in Times of Crisis", 2020.
  14. Felice A. Dacpano, Eli Frazer, and Gönül Vardar-Schara. Characterization of toluene monooxygenases and engineered toluene o-xylene monooxygenases of Pseudomonas sp. OX1 for the metabolism of acetanilide. ACS National Meeting, San Diego, CA, 2022.
  15. Eli Frazer, Felice A. Dacpano, and Gönül Vardar-Schara. Analyzing the potential of toluene o-xylene monooxygenase in acetanilide metabolism by molecular modeling and site directed mutagenesis. ACS National Meeting, San Diego, CA, 2022.
  16. K Cansu Yanık-Yıldırım, Onkar K Phul, Owen S Roth, Areli Tlatelpa, Gerardo Soria-P, Nurcan Vardar-Yel, and Gönül Vardar-Schara. Regiospecific oxidation of chlorobenzene by native and engineered toluene o-xylene monooxygenases. Annual Biomedical Research Conference for Minoritized Scientists, Anaheim, CA, 2022.
  17. Eli Frazer and Gönül Vardar-Schara. Tuning the specificity of toluene o-xylene monooxygenase for acetanilide metabolism. 35th Annual CSU Biotechnology Symposium, Santa Clara, CA, 2023.
  18. Noella Younan, Isabel Gaddo, and Gönül Vardar-Schara. Metabolism of coumarin by toluene monooxygenases and engineered toluene o-xylene monooxygenases of Pseudomonas sp. OX1. 35th Annual CSU Biotechnology Symposium, Santa Clara, CA, 2023.
  19. Eli Frazer and Gönül Vardar-Schara. Regiospecific hydroxylation of acetanilide to acetamidophenols by engineered toluene o-xylene monooxygenases. 27th Annual ACS Green Chemistry & Engineering Conference, Long Beach, CA, 2023.
  20. Noella Younan, Angeline Dauz, and Gönül Vardar-Schara. Application of engineered toluene o-xylene monooxygenases as biocatalysts to generate human metabolite of chlorzoxazone. 36th Annual CSU Biotechnology Symposium, Santa Clara, CA, 2024.

Patent

  1. “Directed Evolution of Recombinant Monooxygenase Polynucleotides and Related Polypeptides and Methods of Use”, Thomas K Wood and Gonul Vardar. 2010. United States Patent US7723498B2.

Updated: January 29, 2024